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Embeds 0 No embeds. No notes for slide. Pcr primer design 1. Primers dictate the successfulness of a PCR Specificity? Proper annealing to the template? Before you start designing primers — Find and use the right resources!
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- Multiplex PCR Primer Design and Analysis!
- A tool for design of primers for microRNA-specific quantitative RT-qPCR.
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After you have your primers designed — Consider a second opinion! It is an updated, task-oriented web-interface to the original Primer3.
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qPCR Assay Design and Optimization
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